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Sunday, April 12, 2009

Special Treatment 2: Assisted Hatching

5:28 PM


Description

Assisted hatching is used when the fertilized egg is wrapped in a membrane (zona pellucida) that is abnormally thick, so much so that the zygote is unable to break the membrane and implant in the uterine wall.


Indications

# The zona pellucida is abnormally thick
# Age (38 or older)
# Have attempted at least 3 times to have a baby using the test-tube method with healthy zygotes and have not conceived
# When the egg or the zygote is cultivated externally, the zona pellucida becomes thicker and harder. If this phenomenon is extreme, the zygote will not be able to hatch for implantation.
# Before the zygote is transferred into the uterus, a microsurgical instrument is used to break the zona pellucida and make hatching and implantation easier.
# Holes can be made with enzymes or acid, or a needle can tear the zona pellucida.


Freeze-preservation

Freeze-preservation: Freezing and preserving the sperm, egg, and zygote, and melting and using them as needed. Preservation Time: Generally 5 years.


Embryo Freezing:

# During the test-tube baby procedure, many egg cells can be collected from induced ovulation, resulting in many zygotes; however, to avoid a multiple pregnancy, the number of embryos transferred is generally limited to 4-5.
# Remaining zygotes are frozen. They are melted and transferred according to the patient's ovulation period, if pregnancy fails during the first transfer period.


Vitrifition Oocyte Freezing:

# The vitrifition method involves soaking the egg in ethyleneglycol for 20 seconds,
# freezing the egg cells (oocytes) very quickly, and preserving them in a jelly state at -196 degrees Celsius.
# As a result, chromosome damage and cytolysis, which can happen during the freezing procedure, are reduced dramatically, and the survival rate and fertilization rate is high, an average of 80-90%.
# Healthy eggs can be freeze-preserved, stored in an egg bank, and melted at a later date for use; patients at risk of premature menopause, career women who want a postpone pregnancy, and cancer patients with dysfunctional ovaries can preserve their eggs and become pregnant when they choose.


IVM-IVF: In Vitro Maturation-In Vitro Fertilization

Premature eggs can be frozen and used after they mature; or the eggs can be fertilized, freeze-preserved, and then used when needed. This method does not require ovulation injections. Premature eggs are collected, externally matured, fertilized, and then the embryos are transferred.


Indications

# Only premature eggs have been collected during the test-tube baby procedure, or eggs are not produced due to early menopause.
# Woman is about to receive radiotherapy or chemotherapy and wishes to harvest her eggs for later use.
# When the test-tube procedure is administered to a polycystic ovary diseased patient with negligible results.
# Sperm can be frozen for later use if it is not possible to collect sperm for artificial insemination or test-tube baby administration. Generally, semen is collected and frozen before cancer treatments or other treatments are initiated that would cause genetic variations in the sperm.

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